mouse strain cre dependent ai32  (Jackson Laboratory)

Journal: Nature Communications

Article Title: An output-null signature of inertial load in motor cortex

doi: 10.1038/s41467-024-51750-7

Figure Lengend Snippet: a Effect of load and motor cortical perturbation on biceps and triceps EMG in a single session. Each contour corresponds to the average step-locked EMG in one of four load and optogenetic perturbation conditions. The angle represents the phase of the step cycle, and the radius of the EMG magnitude at the corresponding step phase. Bold = load, Color = optogenetic perturbation. EMG was normalized in each session based on the distribution of within-step peaks (see Methods). b Effect of load and cerebellar perturbation on biceps and triceps EMG in a single session. c Regression coefficients estimating the effects of load, optogenetic perturbation, the interaction between load and optogenetic perturbation, and step frequency on biceps and triceps EMG (motor cortical perturbation in VGAT-ChR2-EYFP animals: n = 4 mice, n = 18 sessions; cerebellar perturbation in L7Cre-2 x Ai32 animals: n = 3 mice, n = 16 sessions). Each point corresponds to a single experimental session; lines denote 95% confidence intervals. Figure 2a, b adapted from Thompson, E. (2020). Mouse brain above & side. Zenodo. 10.5281/zenodo.3925987. https://creativecommons.org/licenses/by/4.0/ .

Article Snippet: A total of 12 adult mice were used for experiments, including four (male) VGAT-ChR2-EYFP line-8 strain mice (Jackson Laboratory; JAX stock #014548) and eight (six male and two female) L7Cre-2 x Ai32 strain mice (Jackson Laboratory; JAX stock #004146 and #024109).

Techniques:

Journal: Nature Communications

Article Title: An output-null signature of inertial load in motor cortex

doi: 10.1038/s41467-024-51750-7

Figure Lengend Snippet: a Left: mice ( n = 2, L7Cre-2 x Ai32) were chronically implanted with silicon probes in the motor cortex and an optical fiber over the contralateral cerebellar cortex for stimulation of Purkinje cells. Right: raw data showing seven neurons recorded from the motor cortex during locomotion. b Firing rates and spike rasters for six motor cortical neurons recorded across sessions and mice showing different responses to load and cerebellar perturbation in the adaptive locomotion task. Bold = Load, Color = Purkinje cell stimulation. c Effect of load and cerebellar perturbation on motor cortical neurons ( n = 710). Each row corresponds to a single neuron, and displays the difference in z-scored firing rate between load on and off conditions (left) and between Purkinje cell stimulation on and off (right). Neurons are grouped based on the detection of an effect of load (black bar), stimulation (light blue bar), both (dark blue bar), or neither (remaining neurons). d Mean firing rates for all neurons in load on and off conditions (left) and Purkinje cell stimulation on and off (right). Color code reflects the detection of effects of load, stimulation, or both, as in ( c ). Bars indicate 95% confidence intervals for the mean. Figure 3a adapted from Tyler, E., & Kravitz, L. (2020). walking mouse. Zenodo. 10.5281/zenodo.3925915. https://creativecommons.org/licenses/by/4.0/ . Figure 3a adapted from Thompson, E. (2020). Mouse brain above & side. Zenodo. 10.5281/zenodo.3925987. https://creativecommons.org/licenses/by/4.0/ .

Article Snippet: A total of 12 adult mice were used for experiments, including four (male) VGAT-ChR2-EYFP line-8 strain mice (Jackson Laboratory; JAX stock #014548) and eight (six male and two female) L7Cre-2 x Ai32 strain mice (Jackson Laboratory; JAX stock #004146 and #024109).

Techniques: Cell Stimulation

Journal: Nature Communications

Article Title: An output-null signature of inertial load in motor cortex

doi: 10.1038/s41467-024-51750-7

Figure Lengend Snippet: a Left: mice ( n = 6, L7Cre-2 x Ai32) were chronically implanted with fine wire electrodes or Myomatrix electrode arrays in the biceps brachii and triceps brachii muscles and an optical fiber over the ipsilateral cerebellar cortex for stimulation of Purkinje cells. Right: raw motor unit data from the right and left triceps during locomotion recorded from an implanted Myomatrix array. b Firing rates and spike rasters for six spinally-innervated motor units in the adaptive locomotion task. Units 1 and 2 were recorded from the biceps ipsilateral to the load, and units 3–6 from the ipsilateral triceps. Bold = Load, Color = optogenetic perturbation. c Effect of load and cerebellar perturbation on motor units ( n = 108). Each row corresponds to a single motor unit, and displays the difference in z-scored firing rate between load on and off conditions (left) and between Purkinje cell stimulation on and off (right). Units are grouped based on the detection of an effect of load (black bar), stimulation (light blue bar), both (dark blue bar), or neither (remaining neurons). d Mean firing rates for all motor units in load on and off conditions (left) and Purkinje cell stimulation on and off (right). Color code reflects the detection of effects of load, stimulation, or both, as in ( c ). Bars indicate 95% confidence intervals for the mean. Figure 5a adapted from Tyler, E., & Kravitz, L. (2020). walking mouse. Zenodo. 10.5281/zenodo.3925915. https://creativecommons.org/licenses/by/4.0/ . Figure 5a adapted from Thompson, E. (2020). Mouse brain above & side. Zenodo. 10.5281/zenodo.3925987. https://creativecommons.org/licenses/by/4.0/ .

Article Snippet: A total of 12 adult mice were used for experiments, including four (male) VGAT-ChR2-EYFP line-8 strain mice (Jackson Laboratory; JAX stock #014548) and eight (six male and two female) L7Cre-2 x Ai32 strain mice (Jackson Laboratory; JAX stock #004146 and #024109).

Techniques: Muscles, Cell Stimulation